International Journal of Biological Sciences Volume 5
Physiological and Oncogenic Aurora-A Pathway
Toshiaki SAEKI, Mutsuko OUCHI, Toru OUCHI
Authors are encouraged to suggest names of reviewers. Accepted papers
are published immediately. Submissions of previous review reports by other
journals are welcome.
Biolsci.orgAurora family of protein kinases have emerged as crucial factors of, not only mitosis and cytokinesis, but also human carcinogenesis. Among these family members is Aurora-A that is frequently overexpressed in varieties of human cancer. Both in vitro and in vivo studies demonstrated that Aurora-A induces tumorigenesis through genome instability.
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A receptor and binding protein interplay in the detection of a distinct pheromone component in the silkmoth Antheraea polyphemus
Maike Forstner, Heinz Breer, Jürgen Krieger
Male moths respond to conspecific female-released pheromones with remarkable sensitivity and specificity, due to highly specialized chemosensory neurons in their antennae. In Antheraea silkmoths, three types of sensory neurons have been described, each responsive to one of three pheromone components. Since also three different pheromone binding
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
James H. Dyer, Anthony Maina, Iris D. Gomez, Melissa Cadet, Silke Oeljeklaus, Anke C. Schiedel
Thiolase I and II coexist as part of the glyoxysomal β-oxidation system in sunflower (Helianthus annuus L.) cotyledons, the only system shown to have both forms. The importance of thiolases can be underscored not only by their ubiquity, but also by their involvement in a wide variety of processes in plants, animals and bacteria. Here we
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Identification of Anoectochilus based on rDNA ITS sequences alignment and SELDI-TOF-MS
Chuan Gao, Fusheng Zhang, Jun Zhang, Shunxing Guo, Hongbo Shao
The internal transcribed spacer (ITS) sequences alignment and proteomic difference of Anoectochilus interspecies have been studied by means of ITS molecular identification and surface enhanced laser desorption ionization time of flight mass spectrography. Results showed that variety certification on Anoectochilus by ITS sequences can not determine
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A Comparison of the Effects of Three GM Corn Varieties on Mammalian Health
Joël Spiroux de Vendômois, François Roullier, Dominique Cellier, Gilles-Eric Séralini
We present for the first time a comparative analysis of blood and organ system data from trials with rats fed three main commercialized genetically modified (GM) maize (NK 603, MON 810, MON 863), which are present in food and feed in the world. NK 603 has been modified to be tolerant to the broad spectrum herbicide Roundup and thus contains
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The presence of alpha-catenin in the VE-cadherin complex is required for efficient transendothelial migration of leukocytes
Jaap D. van Buul, Floris P. van Alphen, Peter L. Hordijk
The majority of the leukocytes cross the endothelial lining of the vessels through cell-cell junctions. The junctional protein Vascular Endothelial (VE)-cadherin is transiently re-distributed from sites of cell-cell contacts during passage of leukocytes. VE-cadherin is part of a protein complex comprising p120-catenin and beta-catenin as
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Role of Ldb1 in Adult Intestinal Homeostasis
Ipsita Dey-Guha, Mahua Mukhopadhyay, Matthew Phillips, Heiner Westphal
Ldb1 is an essential co-regulator of transcription in embryonic development. It acts in conjunction with nuclear LIM-homeodomain and LIM-only proteins to control key events of organogenesis as precursor cells enter lineage specification. Here we ask whether Ldb1 exerts control over stem cell activation and differentiation throughout the life of
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Proteome Changes in Thai Indigenous Chicken Muscle during Growth Period
Tawatchai Teltathum, Supamit Mekchay
Proteomic profiling of the pectoralis muscle of Thai indigenous chickens during growth period was analyzed using two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/MS). A total of 259, 161, 120 and 107 protein spots were found to be expressed in the chicken
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Azithromycin suppresses interleukin-12p40 expression in lipopolysaccharide and interferon-γ stimulated macrophages
Keiko Yamauchi, Yoko Shibata, Tomomi Kimura, Shuichi Abe, Sumito Inoue, Daisuke Osaka, Michiko Sato, Akira Igarashi, Isao Kubota
Azithromycin (AZM), a 15-member macrolide antibiotic, possesses anti-inflammatory activity. Macrophages are important in innate and acquired immunity, and produce pro-inflammatory cytokines such as interleukin (IL)-12, which are composed of subunit p40 and p35. The key function of IL-12 is the induction and maintenance of T-helper-1 responses,
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Karyology of eight species of bats (Mammalia: Chiroptera) from Hainan Island, China
Yi Wu, Masaharu Motokawa, Yu-Chun Li, Masashi Harada, Zhong Chen, Liang-Kong Lin
Karyotypes and chromosomal data are presented for eight bat species representing two families (Rhinolophidae and Vespertilionidae) from Hainan Island, China. The species investigated were Rhinolophus lepidus (2n = 62, FN = 60), R. pusillus (2n = 62, FN = 60), R. affinis (2n = 62, FN = 60), R. sinicus (2n = 36, FN = 60), Myotis horsfieldi (2n = 44,
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Clinical diagnosis for discogenic low back pain
Yin-gang Zhang, Tuan-mao Guo, Xiong Guo, Shi-xun Wu
Discogenic lower back pain (DLBP) is the most common type of chronic lower back pain (LBP), accounting for 39% of cases, compared to 30% of cases due to disc herniation, and even lower prevalence rates for other causes, such as zygapophysial joint pain. Only a small proportion (approximately 20%) of LBP cases can be attributed with reasonable
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An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL to liver cells through scavenger receptor BI
Xuelei Song, Paul Fischer, Xun Chen, Charlotte Burton, Jun Wang
No more wait for lengthy publication time in other journals only to find that
other researchers/labs have published the same results. Submit your manuscripts
today.
Biolsci.orgApolipoprotein A-I (apoA-I) mimetic peptides have been pursued as new therapeutic agents for the treatment of atherosclerosis, yet their precise mechanism responsible for atheroprotection remains unclear. Like apoA-I itself, most of these peptides are capable of stimulating cholesterol efflux from macrophages or foam cells, and some of them
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Krüppel-like family of transcription factors: an emerging new frontier in fat biology
Christopher W. Brey, Mark P. Nelder, Tiruneh Hailemariam, Randy Gaugler, Sarwar Hashmi
In mammals, adipose tissue stores energy in the form of fat. The ability to regulate fat storage is essential for the growth, development and reproduction of most animals, thus any abnormalities caused by excess fat accumulation can result in pathological conditions which are linked to several interrelated diseases, such as cardiovascular
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Erratum
Zhihua Jiang, Daniel S. Rokhsar, Richard M. Harland
Nucleotide excision repair and recombination are engaged in repair of trans-4-hydroxy-2-nonenal adducts to DNA bases in Escherichia coli
Beata Janowska, Marek Komisarski, Paulina Prorok, Beata Sokołowska, Jarosław Kuśmierek, Celina Janion, Barbara Tudek
One of the major products of lipid peroxidation is trans-4-hydroxy-2-nonenal (HNE). HNE forms highly mutagenic and genotoxic adducts to all DNA bases. Using M13 phage lacZ system, we studied the mutagenesis and repair of HNE treated phage DNA in E. coli wild-type or uvrA, recA, and mutL mutants. These studies revealed that: (i) nucleotide excision
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Failure to detect active virus replication in mast cells at various tissue sites of HIV patients by immunohistochemistry
Ann Marie Nelson, Aaron Auerbach, Yan-gao Man
A recent report postulated that the mast cell population is a significant reservoir for persistent HIV infection. Our study attempted to validate this hypothesis by quantitatively comparing the distribution of mast cells and cells expressing the HIV protein p24 in HIV infected patients. Consecutive sections of paraffin-embedded human tissues from
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Bioinformatics Analysis the Complete Sequences of Cytochrome b of Takydromus sylvaticus and Modeling the Tertiary Structure of Encoded Protein
Qi-Long CHEN, Xin-Sheng TANG, Wen-Juan YAO, Shun-Qing LU
Cytochrome b (cyt b) gene complete sequences (1143bp) of Takydromus sylvaticus were sequenced. In order to clarify the phylogenetic position of the Takydromus sylvaticus, we investigated the phylogeny of 15 Takydromus spp. distributed in East-Asia by Maximum Parsimony (MP), Bayesian Inference (BI), and Maximum Likelihood (ML) methods using DNA
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Fungal Bioconversion of Lignocellulosic Residues; Opportunities & Perspectives
Mehdi Dashtban, Heidi Schraft, Wensheng Qin
The development of alternative energy technology is critically important because of the rising prices of crude oil, security issues regarding the oil supply, and environmental issues such as global warming and air pollution. Bioconversion of biomass has significant advantages over other alternative energy strategies because biomass is the most
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Disruption of the BMEI0066 gene attenuates the virulence of Brucella melitensis and decreases its stress tolerance
Xinglin Zhang, Jie Ren, Na Li, Wenjuan Liu, Qingmin Wu
Brucella melitensis is a facultative intracellular pathogen. An operon composed of BMEI0066, which encodes a two-component response regulator CenR, and BMEI0067, which encodes a cAMP-dependent protein kinase regulatory subunit, has been predicted to exist in many bacterial species. However, little is known about the function of this operon. In
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Comparative characterization of a temperature responsive gene (lactate dehydrogenase-B, ldh-b) in two congeneric tropical fish, Lates calcarifer and Lates niloticus
Richard C. Edmunds, Lynne van Herwerden, Carolyn Smith-Keune, Dean R. Jerry
The characterization of candidate loci is a critical step in obtaining insight into adaptation and acclimation of organisms. In this study of two non-model tropical (to sub-tropical) congeneric perciformes (Lates calcarifer and Lates niloticus) we characterized both coding and non-coding regions of lactate dehydrogenase-B (ldh-b), a locus which
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Protective Effects of Garlic and Silymarin on NDEA-Induced Rats Hepatotoxicity
Sabry M Shaarawy, Amany A Tohamy, Saad M. Elgendy, Zakaria Y Abd Elmageed, Abeer Bahnasy, Maha S Mohamed, Emad Kandil, Khalid Matrougui
Background — The present study was conducted to investigate the chemopreventive effects of garlic extract and silymarin on N-nitrosodiethylamine (NDEA) and carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats. Methods and Results — Animals were pretreated with garlic, silymarin or both for one week prior to the
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A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
Mohamed Rajik, Abdul Rahman Omar, Aini Ideris, Sharifah Syed Hassan, Khatijah Yusoff
Avian influenza viruses (AIV), the causative agent of avian flu or bird flu, cause widespread morbidity and mortality in poultry. The symptoms of the disease range from mild flu like symptoms to death. These viruses possess two important surface glycoproteins, namely hemagglutinin (HA) and neuraminidase (NA) against which neutralizing antibodies
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Discovery of novel genetic networks associated with 19 economically important traits in beef cattle
Zhihua Jiang, Jennifer J. Michal, Jie Chen, Tyler F. Daniels, Tanja Kunej, Matthew D. Garcia, Charles T. Gaskins, Jan R. Busboom, Leeson J. Alexander, Raymond W. Wright Jr., Michael D. MacNeil
Indexed in Medline/PubMed. Full text is beautifully presented and linked.
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biography together with the paper.
Biolsci.orgQuantitative or complex traits are determined by the combined effects of many loci, and are affected by genetic networks or molecular pathways. In the present study, we genotyped a total of 138 mutations, mainly single nucleotide polymorphisms derived from 71 functional genes on a Wagyu x Limousin reference population. Two hundred forty six F2
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Gene expression profiling of chromophobe renal cell carcinomas and renal oncocytomas by Affymetrix GeneChip using pooled and individual tumours
Maria V. Yusenko, Dmitry Zubakov, Gyula Kovacs
Due to overlapping morphology, malignant chromophobe renal cell carcinomas (RCC) and benign renal oncocytomas (RO) may pose a diagnostic problem. In the present study, we have applied different algorithms to evaluate the data sets obtained by hybridisation of pooled and also individual samples of renal cell tumours (RCT) onto two different gene
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The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass
Miranda Maki, Kam Tin Leung, Wensheng Qin
Lignocellulosic biomass is a renewable and abundant resource with great potential for bioconversion to value-added bioproducts. However, the biorefining process remains economically unfeasible due to a lack of biocatalysts that can overcome costly hurdles such as cooling from high temperature, pumping of oxygen/stirring, and, neutralization from
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An apoA-I mimetic peptide increases LCAT activity in mice through increasing HDL concentration
Xun Chen, Charlotte Burton, Xuelei Song, Lesley Mcnamara, Annunziata Langella, Simona Cianetti, Ching H. Chang, Jun Wang
Lecithin cholesterol acyltransferase (LCAT) plays a key role in the reverse cholesterol transport (RCT) process by converting cholesterol to cholesteryl ester to form mature HDL particles, which in turn deliver cholesterol back to the liver for excretion and catabolism. HDL levels in human plasma are negatively correlated with cardiovascular risk
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Lipoproteins, cholesterol homeostasis and cardiac health
Tyler F. Daniels, Karen M. Killinger, Jennifer J. Michal, Raymond W. Wright Jr., Zhihua Jiang
Cholesterol is an essential substance involved in many functions, such as maintaining cell membranes, manufacturing vitamin D on surface of the skin, producing hormones, and possibly helping cell connections in the brain. When cholesterol levels rise in the blood, they can, however, have dangerous consequences. In particular, cholesterol has
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Histopathological effects of cisplatin, doxorubicin and 5-flurouracil (5-FU) on the liver of male albino rats
Hassan I El-Sayyad, Mohamed F Ismail, F M Shalaby, RF Abou-El-Magd, Rajiv L Gaur, Augusta Fernando, Madhwa HG Raj, Allal Ouhtit
Cisplatin, doxorubicin and fluorouracil (5-FU), drugs belonging to different chemical classes, have been extensively used for chemotherapy of various cancers. Despite extensive investigations into their hepatotoxicity, there is very limited information on their effects on the structure and ultra-structure of liver cells in vivo. Here, we
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Site-Dependent Differences in Clinical, Pathohistological, and Molecular Parameters in Metastatic Colon Cancer
Christoph Wilmanns, Sandra Steinhauer, Joachim Grossmann, Günther Ruf
The purpose was to develop a metastatic score specific to the hepatic and peritoneal site in colorectal cancer patients from clinical, pathohistological and molecular markers potentially reflecting oncogenic activation (OA) or epithelial-mesenchymal transition (EMT), where OA may reflect an activation and EMT the functional loss of certain genes.
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Construction of a full-length cDNA Library from Chinese oak silkworm pupa and identification of a KK-42-binding protein gene in relation to pupa-diapause termination
Yu-Ping Li, Run-Xi Xia, Huan Wang, Xi-Sheng Li, Yan-Qun Liu, Zhao-Jun Wei, Cheng Lu, Zhong-Huai Xiang
In this study we successfully constructed a full-length cDNA library from Chinese oak silkworm, Antheraea pernyi, the most well-known wild silkworm used for silk production and insect food. Total RNA was extracted from a single fresh female pupa at the diapause stage. The titer of the library was 5 × 105 cfu/ml and the proportion of
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Essential Roles of mTOR/Akt Pathway in Aurora-A Cell Transformation
Makoto Taga, Eiji Hirooka, Toru Ouchi
We have recently demonstrated that Aurora-A kinase is a potential oncogene to develop mammary gland tumors in mice, when expressed under MMTV promoter. These tumors contain phosphorylated forms of Akt and mTOR, suggesting that Akt-mTOR pathway is involved in transformed phenotype induced by Aurora-A. In the present studies, we discovered that
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How Subchronic and Chronic Health Effects can be Neglected for GMOs, Pesticides or Chemicals
Gilles-Eric Séralini, Joël Spiroux de Vendômois, Dominique Cellier, Charles Sultan, Marcello Buiatti, Lou Gallagher, Michael Antoniou, Krishna R. Dronamraju
Chronic health effects are increasing in the world such as cancers, hormonal, reproductive, nervous, or immune diseases, even in young people. During regulatory toxicological subchronic tests to prevent these on mammalian health, prior commercialization of chemicals, including pesticides and drugs, or GMOs, some statistically significant findings
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Nuclear Localization of p38 MAPK in Response to DNA Damage
C. David Wood, Tina M. Thornton, Guadalupe Sabio, Roger A. Davis, Mercedes Rincon
p38 MAP kinase (MAPK) is activated in response to environmental stress, cytokines and DNA damage, and mediates death, cell differentiation and cell cycle checkpoints. The intracellular localization of p38 MAPK upon activation remains unclear, and may depend on the stimulus. We show here that activation of p38 MAPK by stimuli that induce DNA double
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HCV Antibody Response and Genotype Distribution in Different Areas and Races of China
Leili Jia, Jiyun Yu, Jinliang Yang, Hongbin Song, Xuelin Liu, Yong Wang, Yuanyong Xu, Chuanfu Zhang, Yanwei Zhong, Qiao Li
Leading positions on the Internet, cited by many prestigious
sites, high exposure. Abstract indexed in Medline and PubMed, and full text in
PubMed Central.
Biolsci.orgHepatitis C virus (HCV) heterogeneity accounts for the failure of effective vaccine development and the lack of successful anti-viral therapy in some patients. Little is known about the immune response to HCV peptides and the region or race specific genotypes in China. The objective of this study was to characterize HCV antibody immune response to
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In vivo measurement of protein functional changes
Aili Wang, Zhicheng Zhang, Qinyi Zhao
Conformational changes in proteins are fundamental to all biological functions. In protein science, the concept of protein flexibility is widely used to describe protein dynamics and thermodynamic properties that control protein conformational changes. In this study, we show that urea, which has strong sedative potency, can be administered to fish
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Axial and appendicular skeletal transformations, ligament alterations, and motor neuron loss in Hoxc10 mutants
Sirkka Liisa Hostikka, Jun Gong, Ellen M. Carpenter
Vertebrate Hox genes regulate many aspects of embryonic body plan development and patterning. In particular, Hox genes have been shown to regulate regional patterning of the axial and appendicular skeleton and of the central nervous system. We have identified patterning defects resulting from the targeted mutation of Hoxc10, a member of the Hox10
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BMP13 Prevents the Effects of Annular Injury in an Ovine Model
Aiqun Wei, Lisa A Williams, Divya Bhargav, Bojiang Shen, Thomas Kishen, Neil Duffy, Ashish D Diwan
Chronic back pain is a global health problem affecting millions of people worldwide and carries significant economic and social morbidities. Intervertebral disc damage and degeneration is a major cause of back pain, characterised by histological and biochemical changes that have been well documented in animal models. Recently there has been
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Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina
Mohamed F Ismail, Doaa A Ali, Augusta Fernando, Mohamed E Abdraboh, Rajiv L Gaur, Wael M Ibrahim, Madhwa HG Raj, Allal Ouhtit
Spirulina platensis (SP) is a filamentous cyanobacterium microalgae with potent dietary phyto-antioxidant, anti-inflammatory and anti-cancerous properties. The present study aimed to investigate the chemopreventive effect of SP against rat liver toxicity and carcinogenesis induced by dibutyl nitrosamine (DBN) precursors, and further characterized
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Targeting Promyelocytic Leukemia Protein: A Means to Regulating PML Nuclear Bodies
Erin L. Reineke, Hung-Ying Kao
The promyelocytic leukemia protein (PML) is involved in many cellular processes including cell cycle progression, DNA damage response, transcriptional regulation, viral infection, and apoptosis. These cellular activities often rely on the localization of PML to unique subnuclear structures known as PML nuclear bodies (NBs). More than 50 cellular
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Characterization of the complete mitochondrial genome of the giant silkworm moth, Eriogyna pyretorum (Lepidoptera: Saturniidae)
Shao-Tong Jiang, Gui-Yun Hong, Miao Yu, Na Li, Ying Yang, Yan-Qun Liu, Zhao-Jun Wei
The complete mitochondrial genome (mitogenome) of Eriogyna pyretorum (Lepidoptera: Saturniidae) was determined as being composed of 15,327 base pairs (bp), including 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. The arrangement of the PCGs is the same as that found in the other sequenced lepidopteran. The AT
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THE EXPRESSION OF APELIN AND ITS RECEPTOR APJ DURING DIFFERENT PHYSIOLOGICAL STAGES IN THE BOVINE OVARY
Stefanie Schilffarth, Bernadette Antoni, Dieter Schams, Heinrich HD Meyer, Bajram Berisha
Recent studies implicate that apelin and its receptor APJ may have important role for the modulation of angiogenesis. The aim of this study was to further characterise the regulation of apelin/APJ system in bovine ovary. Experiment 1: corpora lutea (CL) were assigned to the following stages: days 1-2, 3-4, 5-7, 8-12, 13-16, >18 (after
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Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
Wang Ru, Chen Peijie
Purpose: The purpose of this study was to determine whether α-galactosylceramide (α-GalCer), a synthetic glycolipid agonist of natural killer T (NKT) cells, can ameliorate exercise-induced immune imbalance. Methods: Eight-week-old female Sprague-Dawley rats were trained with a progressively increasing load for 9 weeks. At 36 h and at 7 d
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Identification of candidate genes for congenital splay leg in piglets by alternative analysis of DNA microarray data
Steffen Maak, Diana Boettcher, Jens Tetens, Monika Wensch-Dorendorf, Gerd Nürnberg, Klaus Wimmers, Hermann H. Swalve, Georg Thaller
The congenital splay leg syndrome in piglets is characterized by a temporarily impaired functionality of the hind leg muscles immediately after birth. Etiology and pathogenetic mechanisms for the disease are still not well understood. We compared genome wide gene expression of three hind leg muscles (M. adductores, M. gracilis and M. sartorius)
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A male-specific odorant receptor conserved through the evolution of sex pheromones in Ostrinia moth species
Nami Miura, Tatsuro Nakagawa, Sadahiro Tatsuki, Kazushige Touhara, Yukio Ishikawa
In many moths, mate-finding communication is mediated by the female sex pheromones. Since differentiation of sex pheromones is often associated with speciation, it is intriguing to know how the changes in female sex pheromone have been tracked by the pheromone recognition system of the males. A male-specific odorant receptor was found to have been
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Body fat mass reduction and up-regulation of uncoupling protein by novel lipolysis-promoting plant extract
Shinobu Mori, Mayumi Satou, Satoshi Kanazawa, Naonobu Yoshizuka, Tadashi Hase, Ichiro Tokimitsu, Yoshinori Takema, Yoshinori Nishizawa, Toshihiko Yada
Authors are encouraged to suggest names of reviewers. Accepted papers
are published immediately. Submissions of previous review reports by other
journals are welcome.
Biolsci.orgWe have found natural products exhibiting lipolysis-promoting activity in subcutaneous adipocytes, which are less sensitive to hormones than visceral adipocytes. The activities and a action mechanisms of a novel plant extract of Cirsium oligophyllum (CE) were investigated in isolated adipocytes from rat subcutaneous fat, and its fat-reducing
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Vesnarinone Represses the Fibrotic Changes in Murine Lung Injury Induced by Bleomycin
Minoru Inage, Hidenori Nakamura, Hiroshi Saito, Shuichi Abe, Toshihiko Hino, Noriaki Takabatake, Kyoko Terashita, Manabu Ogura, Shuichi Kato, Tetsumi Hosokawa, Makoto Sata, Hitonobu Tomoike
We investigated the potential usefulness of vesnarinone, a novel cytokine inhibitor, for the treatment of lung fibrosis using a murine model of bleomycin (BLM)-induced pulmonary fibrosis. Mice were fed a control diet (n=42), or a diet containing low (n=42) or high (n=42) dose of vesnarinone. Dietary intake of vesnarinone minimized the BLM toxicity
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Old can be new again: HAPPY whole genome sequencing, mapping and assembly
Zhihua Jiang, Daniel S. Rokhsar, Richard M. Harland
During the last three decades, both genome mapping and sequencing methods have advanced significantly to provide a foundation for scientists to understand genome structures and functions in many species. Generally speaking, genome mapping relies on genome sequencing to provide basic materials, such as DNA probes and markers for their
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Characterization of persistent TTX-R Na+ currents in physiological concentration of sodium in rat visceral afferents
Guo-Fen Qiao, Bai-Yan Li, Yu-Hong Zhou, Yan-Jie Lu, John H. Schild
Persistent tetrodotoxin-resistant (TTX-R) Na+ (Nav1.9/SCN11A) currents are not normally recorded in vagal afferent neurons (VANs) with 50 mM of extracellular Na+ although the functional expression of this current was observed in the presence of PGE2 or forskolin. However, it is uncertain whether this current can be seen under physiological
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Responses of Plasma Acetate Metabolism to Hop (Humulus lupulus L.) in Sheep
Mohammad Al-Mamun, Kunio Goto, Sota Chiba, Hiroaki Sano
An isotope dilution method using [1-13C]sodium (Na) acetate was conducted to determine the effect of feeding hop (Humulus lupulus L.) residues on plasma acetate metabolism in six adult crossbred sheep. The sheep were fed 63 g/kg BW0.75/d of either mixed hay (MH-diet) of orchardgrass (Dactylis glomerata L.) and reed canarygrass (Phalaris
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Serum adipokine levels in the obese people
Ilker Tasci, Teoman Dogru, Gokhan Erdem, Serkan Tapan, Cemal Nuri Ercin, Alper Sonmez
Correlated alterations in prostate basal cell layer and basement membrane
Aijun Liu, Lixin Wei, William A. Gardner, Chu-Xia Deng, Yan-Gao Man
Our recent studies revealed that focal basal cell layer disruption (FBCLD) induced auto-immunoreactions represented a contributing factor for human prostate tumor progression and invasion. As the basement membrane surrounds and attaches to the basal cell layer, our current study assessed whether FBCLD would impact the physical integrity of the
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Osteoarthitis of Leptin-Deficient ob/ob Mice in Response to Biomechanical Loading in Micro-CT
Hansjoerg Heep, Gero Hilken, Sebastian Hofmeister, Christian Wedemeyer
Objective: Mechanotransduction is the mechanism that due to reacting chondrocytes on biomechanical loading of body mass. Higher biomechanical loading lead to increased degeneration of chondrocytes, whereas moderate loading is protecting. This suggests that body fat regulates bone metabolism first by means of hormonal factors and second that the
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The Role of SRC-1 in Murine Prostate Carcinogenesis Is Nonessential due to a Possible Compensation of SRC-3/AIB1 Overexpression
Jean Ching-Yi Tien, Suoling Zhou, Jianming Xu
The androgen and androgen receptor (AR)-regulated gene expression plays important roles in normal prostate and prostate cancer development, and AR transcriptional control of genes is mediated by transcriptional coactivators, including the three members of the steroid receptor coactivator (SRC) family, SRC-1 (NCOA1), SRC-2 (TIF2/GRIP1/NCOA2) and
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In vivo evidence of hepato- and reno-protective effect of garlic oil against sodium nitrite-induced oxidative stress
Hanaa A Hassan, Sherif M El-Agmy, Rajiv L Gaur, Augusta Fernando, Madhwa HG Raj, Allal Ouhtit
Sodium nitrite (NaNO2), a food color fixative and preservative, contributes to carcinogenesis. We investigated the protective role of garlic oil against NaNO2-induced abnormalities in metabolic biochemical parameters and oxidative status in male albino rats. NaNO2 treatment for a period of three months induced a significant increase in serum
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Birth weight and coronary artery disease. The effect of gender and diabetes
Maria Banci, Patrizia Saccucci, Alessandro Dofcaci, Ilaria Sansoni, Andrea Magrini, Egidio Bottini, Fulvia Gloria-Bottini
Background: The developmental origin theory of coronary heart disease proposes that undernutrition in utero permanently changes body functions and metabolism leading to an increased risk of coronary artery diseases (CAD) in adult life. Some studies support this theory but others suggest that birth weight (BW) is not a major risk factor for
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A Curriculum Vitae of Teeth: Evolution, Generation, Regeneration
Despina S. Koussoulakou, Lukas H. Margaritis, Stauros L. Koussoulakos
Leading positions on the Internet, cited by many prestigious
sites, high exposure. Abstract indexed in Medline and PubMed, and full text in
PubMed Central.
Biolsci.orgThe ancestor of recent vertebrate teeth was a tooth-like structure on the outer body surface of jawless fishes. Over the course of 500,000,000 years of evolution, many of those structures migrated into the mouth cavity. In addition, the total number of teeth per dentition generally decreased and teeth morphological complexity increased. Teeth form
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Diverse protein regulations on PHA formation in Ralstonia eutropha on short chain organic acids
Sung-Eun Lee, Qing X. Li, Jian Yu
Organic acids are considered as potential substrates for biosynthesis of polyhydroxyalkaonates. The acids may also be the metabolic inhibitors at moderate concentration levels. In this study, Ralstonia eutropha was used to elucidate the protein regulations when the bacterial cells pre-cultivated on glucose were exposed to three representative
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Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei
Yanisa Laoong-u-thai, Baoping Zhao, Amornrat Phongdara, Harry Ako, Jinzeng Yang
Small ubiquitin-like modifiers (SUMO) work in a similar way as ubiquitin to alter the biological properties of a target protein by conjugation. A shrimp SUMO cDNA named LvSUMO-1 was identified in Litopenaeus vannamei. LvSUMO-1 cDNA contains a coding sequence of 282 nucleotides with untranslated regions of 37 bp at 5'-end and 347 bp at 3'-end,
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Is sodium current present in human sinoatrial node cells?
Arie O. Verkerk, Ronald Wilders, Marcel M.G.J. van Borren, Hanno L. Tan
Pacemaker activity of the sinoatrial node has been studied extensively in various animal species, but is virtually unexplored in man. As such, it is unknown whether the fast sodium current (INa) plays a role in the pacemaker activity of the human sinoatrial node. Recently, we had the unique opportunity to perform patch-clamp experiments on single
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BMP-13 Emerges as a Potential Inhibitor of Bone Formation
Bojiang Shen, Divya Bhargav, Aiqun Wei, Lisa A Williams, Helen Tao, David D F Ma, Ashish D Diwan
Bone morphogenetic protein-13 (BMP-13) plays an important role in skeletal development. In the light of a recent report that mutations in the BMP-13 gene are associated with spine vertebral fusion in Klippel-Feil syndrome, we hypothesized that BMP-13 signaling is crucial for regulating embryonic endochondral ossification. In this study, we found
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Association between Apoptotis and CD4+/CD8+ T-Lymphocyte Ratio in Aseptic Loosening after Total Hip Replacement
Stefan Landgraeber, Marius von Knoch, Franz Löer, Jochen Brankamp, Michael Tsokos, Florian Grabellus, Kurt Werner Schmid, Martin Totsch
Particle-induced osteolysis is a major cause of aseptic loosening after total joint replacement. While the osteolytic cascade initiated by cytokine release from macrophages has been studied extensively, the involvement of T-lymphocytes in this context is controversial and has been addressed by only a few authors. In a former study we detected that
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Developing tTA Transgenic Rats for Inducible and Reversible Gene Expression
Hongxia Zhou, Cao Huang, Min Yang, Carlisle P Landel, Pedro Yuxing Xia, Yong-Jian Liu, Xu Gang Xia
To develop transgenic lines for conditional expression of desired genes in rats, we generated several lines of the transgenic rats carrying the tetracycline-controlled transactivator (tTA) gene. Using a vigorous, ubiquitous promoter to drive the tTA transgene, we obtained widespread expression of tTA in various tissues. Expression of tTA was
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Understanding Quantitative Genetics in the Systems Biology Era
Mengjin Zhu, Mei Yu, Shuhong Zhao
Biology is now entering the new era of systems biology and exerting a growing influence on the future development of various disciplines within life sciences. In early classical and molecular periods of Biology, the theoretical frames of classical and molecular quantitative genetics have been systematically established, respectively. With the new
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Activity of Chitosans in combination with antibiotics in Pseudomonas aeruginosa
San Tin, Kishore R. Sakharkar, Chu Sing Lim, Meena K. Sakharkar
Chitosan and its derivative water soluble Chitosan oligosaccharide are used in a variety of applications in pharmaceutical preparations. In this study, 2 wild (ATCC 15729 and PAO1) and 2 mutant strains (PT121 and PT149) of P. aeruginosa are investigated for drug-drug interactions in vitro. 10 antimicrobial agents (antibiotics) are combined with
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SIRT1, Is It a Tumor Promoter or Tumor Suppressor?
Chu-Xia Deng
SIRT1 has been considered as a tumor promoter because of its increased expression in some types of cancers and its role in inactivating proteins that are involved in tumor suppression and DNA damage repair. However, recent studies demonstrated that SIRT1 levels are reduced in some other types of cancers, and that SIRT1 deficiency results in
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Antitumor Activity of T Cells Generated from Lymph Nodes Draining the SEA-expressing Murine B16 Melanoma and Secondarily Activated with Dendritic Cells
Jiyun Yu, Rong Tian, Bingshui Xiu, Jinqi Yan, Rui Jia, Liang Zhang, Alfred E. Chang, Hongbin Song, Qiao Li
The successful use of tumor-draining lymph nodes (TDLN) as a source of effector cells for cancer immunotherapy depends largely on the immunogenicity of the tumor drained by the lymph nodes as well as the methods for secondary in vitro T cell activation and expansion. We transferred the bacterial superantigen staphylococcal enterotoxin A (SEA) gene
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Assay Validation for KIM-1: human urinary renal dysfunction biomarker
Shalini Chaturvedi, Takeisha Farmer, Gordon F. Kapke
Leading positions on the Internet, cited by many prestigious
sites, high exposure. Abstract indexed in Medline and PubMed, and full text in
PubMed Central.
Biolsci.orgUrinary kidney injury molecule (KIM-1) is a sensitive quantitative biomarker for early detection of kidney tubular injury. The objective of the present work was to analytically validate this urinary renal injury biomarker. Duo-set reagents from R&D were used to develop the ELISA and validate the assay's linearity, intra-run precision,
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Differential expression of neurotrophins in postnatal C57BL/6 mice striatum
V. Zermeño, S. Espindola, E. Mendoza, E. Hernández-Echeagaray
Neurotrophin expression in early stages of development is crucial for brain assembly and function. In particular, postnatal expression of neurotrophins has not been well documented in the neostriatum and in general neurotrophins or their receptor mRNA's are normally reported, but not protein expression. In the present study, immunocytochemical
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The Fascinating World of RNA Interference
Afsar Raza Naqvi, Md. Nazrul Islam, Nirupam Roy Choudhury, Qazi Mohd. Rizwanul Haq.
Micro- and short-interfering RNAs represent small RNA family that are recognized as critical regulatory species across the eukaryotes. Recent high-throughput sequencing have revealed two more hidden players of the cellular small RNA pool. Reported in mammals and Caenorhabditis elegans respectively, these new small RNAs are named piwi-interacting
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Aberrant p63 and WT-1 expression in myoepithelial cells of pregnancy-associated breast cancer: implications for tumor aggressiveness and invasiveness
Zheli Xu, Wan Wang, Chu-Xia Deng, Yan-gao Man
Our recent studies revealed that focal alterations in breast myoepithelial cell layers significantly impact the biological presentation of associated epithelial cells. As pregnancy-associated breast cancer (PABC) has a significantly more aggressive clinical course and mortality rate than other forms of breast malignancies, our current study
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STAT2*C related genotypes and allele but not TLR4 and CD40 gene polymorphisms are associated with higher susceptibility for asthma
Yao-Yuan Hsieh, Lei Wan, Chi-Chen Chang, Chang-Hai Tsai, Fuu-Jen Tsai
Objective: Asthma is caused by a complex interaction between multiple genes and environmental factors. Herein we aimed to investigate whether signal transducer and activator of transcription (STAT2), toll-like receptors 4 (TLRs4) and CD40-related polymorphisms are associated with asthma susceptibility. Design: Children were divided: (1) asthma
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SpolvlgA is a DDX3/PL10-related DEAD-box RNA helicase expressed in blastomeres and embryonic cells in planarian embryonic development
Jordi Solana, Rafael Romero
Planarian flatworms have an impressive regenerative power. Although their embryonic development is still poorly studied and is highly derived it still displays some simple characteristics. We have identified SpolvlgA, a Schmidtea polychroa homolog of the DDX3/PL10 DEAD-box RNA helicase DjvlgA from the planarian species Dugesia japonica. This gene
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Free tyrosine and tyrosine-rich peptide-dependent superoxide generation catalyzed by a copper-binding, threonine-rich neurotoxic peptide derived from prion protein
Ken Yokawa, Tomoko Kagenishi, Kaishi Goto, Tomonori Kawano
Previously, generation of superoxide anion (O2•-) catalyzed by Cu-binding peptides derived from human prion protein (model sequence for helical Cu-binding motif VNITKQHTVTTTT was most active) in the presence of catecholamines and related aromatic monoamines such as phenylethylamine and tyramine, has been reported [Kawano, T., Int J Biol Sci
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Non-Classical P38 Map Kinase Functions: Cell Cycle Checkpoints and Survival
Tina M. Thornton, Mercedes Rincon
The p38 MAPK kinase pathway is activated in response to a wide range of cellular stress stimuli and cytokines. Our understanding of the important functions of p38 MAPK in the process of differentiation and cell death has grown considerably in the recent years and is now relatively established. Here we discuss the role of p38 MAPK in the mediation
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Molecular cloning and characterization of pigeon (Columba liva) ubiquitin and ubiquitin-conjugating enzyme genes from pituitary gland library
Peng-fei Gao, Guo-qing Cao, Hui-ting Zhao, Gui-xian Zhang, Yu-suo Jiang, Qin-de Wang
In the study of the regulation of incubation, broodiness and laying performance in pigeons (Columba liva), a cDNA library, which was enriched with full-length brooding-related genes, was constructed by SMART LD-PCR techniques using the pituitary glands of incubating White King pigeons. The titers of optimal primary libraries were 1.54×106
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Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition
Xiuxing Wang, Chunyang Xue, Xiaona Wang, Honglin Liu, Yinxue Xu, Ruqian Zhao, Zhihua Jiang, Michael V. Dodson, Jie Chen
Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between longissimus dorsi (LD) muscles with extremely different IMF content. A major DE gene associated with
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BRCA1, Hormone, and Tissue-Specific Tumor Suppression
Yanfen Hu
Germline mutations of BRCA1 predispose women to breast and ovarian cancers. Elucidating molecular mechanism of tissue- and gender-specific phenomena in BRCA1-related tumors is a key to our understanding of BRCA1 function in tumor suppression. This review summarizes studies in recent years on the link between BRCA1 and estrogen/progesterone
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Using Profiles Based on Nucleotide Hydrophobicity to Define Essential Regions for Splicing
Galina Boldina, Anatoly Ivashchenko, Mireille Régnier
Indexed in Medline/PubMed. Full text is beautifully presented and linked.
High exposure on the Internet and search engines. Authors can also publish a
biography together with the paper.
Biolsci.orgThe splice-site sequences of U2-type introns are highly degenerate, so many different sequences can function as U2-type splice sites. Using our new profiles based on hydrophobicity properties we pointed out specific properties for regions surrounding splice sites. We built a set T of flanking regions of genes with 1-3 introns from 21st and 22nd
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Genome-wide Analysis of BP1 Transcriptional Targets in Breast Cancer Cell Line Hs578T
Yongchun Song, Chengxue Dang, Yebo Fu, Yi Lian, Jenny Hottel, Xuelan Li, Tim McCaffrey, Sidney W. Fu
Homeobox genes are known to be critically important in tumor development and progression. The BP1 (Beta Protein 1) gene, an isoform of DLX4, belongs to the Distal-less (DLX) subfamily of homeobox genes and encodes a homeodomain-containing transcription factor. Our studies have shown that the BP1 gene was overexpressed in 81% of primary breast
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